Question about trimming drone for autonomous flight

Good morning,

We're working on a school project that has a drone flying autonomously using dronekit scripts. None of us are very experienced with flying drones so we're definitely going through the props. My question is, is it absolutely necessary to trim the drone perfectly in stabilize mode before we run our scripts in guided mode? Does trimming it in stabilize mode affect how it flies in guided mode? Or are these two things not connected? 

Any help would be great!

Thanks!

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